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zebrafish codon optimized cas9 coding sequence  (Addgene inc)


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    Addgene inc zebrafish codon optimized cas9 coding sequence
    Zebrafish Codon Optimized Cas9 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zebrafish+codon+optimized+cas9+coding+sequence/PCS2%2B+Cas9+(Plasmid+%23122948)/hamar_jens_carlton__2024__the_myo_inositol_biosynthesis_pathway_and_its_regulation_by_nfat5_in_tilapia_salinity_tolerance-298-1-10
    Average 93 stars, based on 49 article reviews
    zebrafish codon optimized cas9 coding sequence - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: The Myo-inositol Biosynthesis Pathway and its Regulation by NFAT5 in Tilapia Salinity Tolerance Assessed by Genetic Manipulation of Cultured Cells
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Article Title: An Efficient Vector-based CRISPR/Cas9 System in an Oreochromis mossambicus Cell Line using Endogenous Promoters
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Article Title: An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Plasmid Preparation:

    Article Title: The Myo-inositol Biosynthesis Pathway and its Regulation by NFAT5 in Tilapia Salinity Tolerance Assessed by Genetic Manipulation of Cultured Cells
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Article Title: An Efficient Vector-based CRISPR/Cas9 System in an Oreochromis mossambicus Cell Line using Endogenous Promoters
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Article Title: An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Clone Assay:

    Article Title: The Myo-inositol Biosynthesis Pathway and its Regulation by NFAT5 in Tilapia Salinity Tolerance Assessed by Genetic Manipulation of Cultured Cells
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Article Title: An Efficient Vector-based CRISPR/Cas9 System in an Oreochromis mossambicus Cell Line using Endogenous Promoters
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Article Title: An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Polymerase Chain Reaction:

    Article Title: The Myo-inositol Biosynthesis Pathway and its Regulation by NFAT5 in Tilapia Salinity Tolerance Assessed by Genetic Manipulation of Cultured Cells
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Article Title: An Efficient Vector-based CRISPR/Cas9 System in an Oreochromis mossambicus Cell Line using Endogenous Promoters
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Article Title: An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Amplification:

    Article Title: The Myo-inositol Biosynthesis Pathway and its Regulation by NFAT5 in Tilapia Salinity Tolerance Assessed by Genetic Manipulation of Cultured Cells
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Article Title: An Efficient Vector-based CRISPR/Cas9 System in an Oreochromis mossambicus Cell Line using Endogenous Promoters
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Article Title: An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Construct:

    Article Title: The Myo-inositol Biosynthesis Pathway and its Regulation by NFAT5 in Tilapia Salinity Tolerance Assessed by Genetic Manipulation of Cultured Cells
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Article Title: An Efficient Vector-based CRISPR/Cas9 System in an Oreochromis mossambicus Cell Line using Endogenous Promoters
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).

    Article Title: An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters
    Article Snippet: The OmEF1aCas9P2ApuroSB vector was constructed using the pSBbi-GP (Addgene # 60511) plasmid as the base vector. .. The zebrafish codon optimized Cas9 coding sequence from the pCS2-nCas9n (Addgene # 47929) plasmid and a puromycin resistance gene were cloned downstream of the OmEF1a promoter PCR amplicon separated by a P2A self-cleaving peptide into the base vector between the ITRs to complete the construct. .. The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).



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    zebrafish codon optimized cas9 coding sequence  (Addgene inc)
    93
    Addgene inc zebrafish codon optimized cas9 coding sequence
    Zebrafish Codon Optimized Cas9 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zebrafish+codon+optimized+cas9+coding+sequence/PCS2%2B+Cas9+(Plasmid+%23122948)/hamar_jens_carlton__2024__the_myo_inositol_biosynthesis_pathway_and_its_regulation_by_nfat5_in_tilapia_salinity_tolerance-298-1-10
    Average 93 stars, based on 1 article reviews
    zebrafish codon optimized cas9 coding sequence - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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